LATE-PCR

Smiths Detection uses an advanced PCR chemistry called Linear After The Exponential PCR or LATE-PCR. LATE-PCR is an asymmetric PCR chemistry developed at Prof Larry Wangh’s laboratory at Brandeis University.

LATE-PCR is a powerful PCR chemistry enabling users to carry out assays with much greater discriminatory power than has typically been available to date. LATE-PCR can be highly multiplexed to allow the detection and identification of a wide range of targets in a single assay. As an asymmetric PCR chemistry the products are available for direct sequencing without the common clean up procedures needed to remove primers etc. In addition, LATE-PCR as used in our instruments at end point, allows the automatic identification of a positive or negative identification of a sample.

A detailed review of the LATE-PCR chemistry and its application has recently been published in Nature Methods. More information about LATE-PCR can be obtained from the references below.
Website
http://www.late-pcr.com/
Selected Publications
John E Rice, J Aquiles Sanchez, Kenneth E Pierce, Arthur H Reis Jr. Adam Osborne & Lawrence Wangh (2007) Monoplex/multiplex linear-after-the-exponential-PCR assays combined with PrimeSafe and Dilute-'N'-Go sequencing. Nature Protocols, *2, *#10,* *2429-2438

Pierce, K.E., Sanchez, J.A., Rice, J.E., and Wangh, L.J. (2005) Linear-After-The-Exponential (LATE)-PCR: Primer design criteria for high yields of specific single-stranded DNA and improved real-time detection, Proc Natl Acad Sci USA, 102:8609-8614.
Sanchez, J.A., Pierce, K.E., Rice, J.E., and Wangh, L.J. (2004) Linear-After-The-Exponential (LATE)-PCR: An advanced method of asymmetric PCR and its uses in quantitative real-time analysis, Proc Natl Acad Sci USA, 101(7):1933-1938.